Xenocin Export by the Flagellar Type III Pathway in Xenorhabdus nematophila
نویسندگان
چکیده
منابع مشابه
Xenocin export by the flagellar type III pathway in Xenorhabdus nematophila.
The xenocin operon of Xenorhabdus nematophila consists of xciA and ximB genes encoding a 64-kDa xenocin and 42-kDa immunity protein to kill competing microbes in the insect larva. The catalytic domain of xenocin has RNase activity and is responsible for its cytotoxicity. Under SOS conditions, xenocin is produced with immunity protein as a complex. Here, we show that xenocin and immunity protein...
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Flagella are assembled sequentially from the inside-out with morphogenetic checkpoints that enforce the temporal order of subunit addition. Here we show that flagellar basal bodies fail to proceed to hook assembly at high frequency in the absence of the monotopic protein SwrB of Bacillus subtilis. Genetic suppressor analysis indicates that SwrB activates the flagellar type III secretion export ...
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T he gram-negative bacterium Xenorhabdus nematophila engages in a mutualistic relationship with a specific soil nematode and also can mount potent pathogenic attacks on a variety of insects. During its mutualisticpathogenic life cycle, Xenorhabdus produces a wide range of exoenzymes, antimicrobial and nematicidal compounds, and insect toxins. Moreover, the nematode-bacteria complex is useful fo...
متن کاملExpression, Purification, and Functional Characterization of Atypical Xenocin, Its Immunity Protein, and Their Domains from Xenorhabdus nematophila
Xenorhabdus nematophila, a gram-negative bacterium belonging to the family Enterobacteriaceae is a natural symbiont of a soil nematode from the family Steinernematidae. In this study cloning, expression, and purification of broad range iron regulated multidomain bacteriocin called xenocin from X. nematophila (66 kDa, encoded by xcinA gene) and its multidomain immunity protein (42 kDa, encoded b...
متن کاملTranscriptional analysis and functional characterization of a gene pair encoding iron-regulated xenocin and immunity proteins of Xenorhabdus nematophila.
We describe a two-gene cluster encoding a bacteriocin, xenocin, and the cognate immunity protein in the insect-pathogenic bacterium Xenorhabdus nematophila, which infects and kills larval stages of the common crop pest Helicoverpa armigera. The two genes, xcinA and ximB, are present in the genome as a single transcriptional unit, which is regulated under SOS conditions. The stress-inducible pro...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2013
ISSN: 0021-9193
DOI: 10.1128/jb.01532-12